WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... CTGGACAATCAGAAAGAGATTGCCGAGATGCAAAATGAGACTCAAAAAGAGATTGCTGGC Read1 before filtering: total reads: 4684181 total bases: 674522064 Q20 bases: 654718910(97.0641%) Q30 bases: 628012121(93.1048%) Read1 after filtering: total reads: 4655803 total bases: 670399245 Q20 bases: 651358866(97.1598%) Q30 bases: 625001261(93.2282%) Filtering result: reads passed filter: 4655803 reads failed due to low quality: 8136 reads failed due to too many N: 2827 reads failed due to too short: 17415 reads with adapter trimmed: 22941 bases trimmed due to adapters: 2541573 Duplication rate (may be overestimated since this is SE data): 71.1335% JSON report: CypressIslandWA_FG145.json HTML report: CypressIslandWA_FG145.html fastp -i CypressIslandWA_FG145.F.fq.gz -o CypressIslandWA_FG145.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG145.json -h CypressIslandWA_FG145.html fastp v0.20.0, time used: 43 seconds