WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... ATAAGGCCACGTATTTTGCAAGCTATTTAACTGGCGGCGATTGCGTACCCGACGACCAAA Read1 before filtering: total reads: 3284610 total bases: 472983840 Q20 bases: 459064611(97.0571%) Q30 bases: 440193743(93.0674%) Read1 after filtering: total reads: 3259921 total bases: 469392473 Q20 bases: 456035650(97.1544%) Q30 bases: 437439757(93.1928%) Filtering result: reads passed filter: 3259921 reads failed due to low quality: 5914 reads failed due to too many N: 1965 reads failed due to too short: 16810 reads with adapter trimmed: 22744 bases trimmed due to adapters: 2455325 Duplication rate (may be overestimated since this is SE data): 72.0109% JSON report: CypressIslandWA_FG144.json HTML report: CypressIslandWA_FG144.html fastp -i CypressIslandWA_FG144.F.fq.gz -o CypressIslandWA_FG144.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG144.json -h CypressIslandWA_FG144.html fastp v0.20.0, time used: 30 seconds