WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
ATAAAGGAAAGGATACTCGTGATTATCTTGCTGCTGCATTTCCTGAGCTTAATGCTTGGG

Read1 before filtering:
total reads: 2756398
total bases: 396921312
Q20 bases: 385739437(97.1828%)
Q30 bases: 370381399(93.3136%)

Read1 after filtering:
total reads: 2739020
total bases: 394351909
Q20 bases: 383614173(97.2771%)
Q30 bases: 368464789(93.4355%)

Filtering result:
reads passed filter: 2739020
reads failed due to low quality: 4664
reads failed due to too many N: 1644
reads failed due to too short: 11070
reads with adapter trimmed: 21297
bases trimmed due to adapters: 1659777

Duplication rate (may be overestimated since this is SE data): 72.8202%

JSON report: CypressIslandWA_FG143.json
HTML report: CypressIslandWA_FG143.html

fastp -i CypressIslandWA_FG143.F.fq.gz -o CypressIslandWA_FG143.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG143.json -h CypressIslandWA_FG143.html 
fastp v0.20.0, time used: 27 seconds