WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4371885
total bases: 629551440
Q20 bases: 608990264(96.734%)
Q30 bases: 583314902(92.6556%)

Read1 after filtering:
total reads: 4361693
total bases: 628080141
Q20 bases: 608186701(96.8327%)
Q30 bases: 582759889(92.7843%)

Filtering result:
reads passed filter: 4361693
reads failed due to low quality: 7590
reads failed due to too many N: 2602
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 73.5576%

JSON report: CypressIslandWA_FG141.json
HTML report: CypressIslandWA_FG141.html

fastp -i CypressIslandWA_FG141.F.fq.gz -o CypressIslandWA_FG141.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG141.json -h CypressIslandWA_FG141.html 
fastp v0.20.0, time used: 48 seconds