WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3856367
total bases: 555316848
Q20 bases: 538773080(97.0208%)
Q30 bases: 517089299(93.1161%)

Read1 after filtering:
total reads: 3847215
total bases: 553996844
Q20 bases: 538048765(97.1213%)
Q30 bases: 516587520(93.2474%)

Filtering result:
reads passed filter: 3847215
reads failed due to low quality: 6825
reads failed due to too many N: 2327
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 72.7002%

JSON report: CypressIslandWA_FG139.json
HTML report: CypressIslandWA_FG139.html

fastp -i CypressIslandWA_FG139.F.fq.gz -o CypressIslandWA_FG139.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG139.json -h CypressIslandWA_FG139.html 
fastp v0.20.0, time used: 44 seconds