WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
GGCCTCCACTATGAAATCGCGTAGAGGCTTTGCTATTCAGCGTTTGATGAATGCAATGCG

Read1 before filtering:
total reads: 2769297
total bases: 398778768
Q20 bases: 388053369(97.3104%)
Q30 bases: 373228476(93.5929%)

Read1 after filtering:
total reads: 2755395
total bases: 396770208
Q20 bases: 386486599(97.4082%)
Q30 bases: 371858281(93.7213%)

Filtering result:
reads passed filter: 2755395
reads failed due to low quality: 4750
reads failed due to too many N: 1593
reads failed due to too short: 7559
reads with adapter trimmed: 8388
bases trimmed due to adapters: 1093660

Duplication rate (may be overestimated since this is SE data): 73.1626%

JSON report: CypressIslandWA_FG138.json
HTML report: CypressIslandWA_FG138.html

fastp -i CypressIslandWA_FG138.F.fq.gz -o CypressIslandWA_FG138.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG138.json -h CypressIslandWA_FG138.html 
fastp v0.20.0, time used: 27 seconds