WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... GAAATGAAGACGGCCATTAGCTGTACCATACTCAGGCACACAAAAATACTGATAGCAGTC Read1 before filtering: total reads: 2478314 total bases: 356877216 Q20 bases: 346587750(97.1168%) Q30 bases: 332535884(93.1794%) Read1 after filtering: total reads: 2460464 total bases: 354235864 Q20 bases: 344351394(97.2096%) Q30 bases: 330503789(93.3005%) Filtering result: reads passed filter: 2460464 reads failed due to low quality: 4147 reads failed due to too many N: 1460 reads failed due to too short: 12243 reads with adapter trimmed: 23272 bases trimmed due to adapters: 1832189 Duplication rate (may be overestimated since this is SE data): 67.6414% JSON report: CypressIslandWA_FG135.json HTML report: CypressIslandWA_FG135.html fastp -i CypressIslandWA_FG135.F.fq.gz -o CypressIslandWA_FG135.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG135.json -h CypressIslandWA_FG135.html fastp v0.20.0, time used: 23 seconds