WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2173945
total bases: 313048080
Q20 bases: 301280951(96.2411%)
Q30 bases: 281636018(89.9657%)

Read1 after filtering:
total reads: 2169238
total bases: 312278183
Q20 bases: 300923061(96.3638%)
Q30 bases: 281429893(90.1215%)

Filtering result:
reads passed filter: 2169238
reads failed due to low quality: 4053
reads failed due to too many N: 654
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 31.4956%

JSON report: CypressIslandWA_FG134.json
HTML report: CypressIslandWA_FG134.html

fastp -i CypressIslandWA_FG134.F.fq.gz -o CypressIslandWA_FG134.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG134.json -h CypressIslandWA_FG134.html 
fastp v0.20.0, time used: 31 seconds