WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
AAGGCGCATAACGATACCACTGACCCTCAGCAATCTTAAACTTCTTAGACGAATCACCAG

Read1 before filtering:
total reads: 2813022
total bases: 405075168
Q20 bases: 393518532(97.147%)
Q30 bases: 377497365(93.1919%)

Read1 after filtering:
total reads: 2790306
total bases: 401144436
Q20 bases: 390085076(97.243%)
Q30 bases: 374348929(93.3202%)

Filtering result:
reads passed filter: 2790306
reads failed due to low quality: 4753
reads failed due to too many N: 1703
reads failed due to too short: 16260
reads with adapter trimmed: 32299
bases trimmed due to adapters: 2999335

Duplication rate (may be overestimated since this is SE data): 65.9513%

JSON report: CypressIslandWA_FG132.json
HTML report: CypressIslandWA_FG132.html

fastp -i CypressIslandWA_FG132.F.fq.gz -o CypressIslandWA_FG132.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG132.json -h CypressIslandWA_FG132.html 
fastp v0.20.0, time used: 28 seconds