WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
AAAAGCGGCATGGTCAATATAACCAGTAGTGTTAACAGTCGGGAGAGGAGTGGCATTAAC

Read1 before filtering:
total reads: 2557723
total bases: 368312112
Q20 bases: 358419361(97.314%)
Q30 bases: 344572382(93.5545%)

Read1 after filtering:
total reads: 2535587
total bases: 365087973
Q20 bases: 355564315(97.3914%)
Q30 bases: 341923319(93.655%)

Filtering result:
reads passed filter: 2535587
reads failed due to low quality: 3521
reads failed due to too many N: 1542
reads failed due to too short: 17073
reads with adapter trimmed: 23235
bases trimmed due to adapters: 2493258

Duplication rate (may be overestimated since this is SE data): 69.9578%

JSON report: CypressIslandWA_FG131.json
HTML report: CypressIslandWA_FG131.html

fastp -i CypressIslandWA_FG131.F.fq.gz -o CypressIslandWA_FG131.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG131.json -h CypressIslandWA_FG131.html 
fastp v0.20.0, time used: 31 seconds