WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3853785
total bases: 554945040
Q20 bases: 536143656(96.612%)
Q30 bases: 510929382(92.0685%)

Read1 after filtering:
total reads: 3845574
total bases: 553759388
Q20 bases: 535486764(96.7003%)
Q30 bases: 510475578(92.1836%)

Filtering result:
reads passed filter: 3845574
reads failed due to low quality: 5997
reads failed due to too many N: 2214
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 67.287%

JSON report: CypressIslandWA_FG130.json
HTML report: CypressIslandWA_FG130.html

fastp -i CypressIslandWA_FG130.F.fq.gz -o CypressIslandWA_FG130.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG130.json -h CypressIslandWA_FG130.html 
fastp v0.20.0, time used: 53 seconds