WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
CTTTACGCTTGCCTTTAGTACCTCGCAACGGCTGCGGACGACCAGGGCGAGCGCCAGAAC

Read1 before filtering:
total reads: 2992620
total bases: 430937280
Q20 bases: 419037057(97.2385%)
Q30 bases: 402583655(93.4205%)

Read1 after filtering:
total reads: 2973079
total bases: 427785178
Q20 bases: 416405652(97.3399%)
Q30 bases: 400228195(93.5582%)

Filtering result:
reads passed filter: 2973079
reads failed due to low quality: 5090
reads failed due to too many N: 1836
reads failed due to too short: 12615
reads with adapter trimmed: 23240
bases trimmed due to adapters: 2153354

Duplication rate (may be overestimated since this is SE data): 69.0045%

JSON report: CypressIslandWA_FG129.json
HTML report: CypressIslandWA_FG129.html

fastp -i CypressIslandWA_FG129.F.fq.gz -o CypressIslandWA_FG129.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG129.json -h CypressIslandWA_FG129.html 
fastp v0.20.0, time used: 29 seconds