WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
TATTGGTATCAGGGTTAATCGTGCCAAGAAAAGCGGCATGGTCAATATAACCAGTAGTGT

Read1 before filtering:
total reads: 3373318
total bases: 485757792
Q20 bases: 471882981(97.1437%)
Q30 bases: 452939994(93.244%)

Read1 after filtering:
total reads: 3347894
total bases: 482044887
Q20 bases: 468735919(97.2391%)
Q30 bases: 450066765(93.3662%)

Filtering result:
reads passed filter: 3347894
reads failed due to low quality: 5964
reads failed due to too many N: 1973
reads failed due to too short: 17487
reads with adapter trimmed: 25789
bases trimmed due to adapters: 2568492

Duplication rate (may be overestimated since this is SE data): 66.1677%

JSON report: CypressIslandWA_FG128.json
HTML report: CypressIslandWA_FG128.html

fastp -i CypressIslandWA_FG128.F.fq.gz -o CypressIslandWA_FG128.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG128.json -h CypressIslandWA_FG128.html 
fastp v0.20.0, time used: 36 seconds