WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
AGAAGACTCAAAGCGAACCAAACAGGCAAAAAATTTAGGGTCGGCATCAAAAGCAATATC

Read1 before filtering:
total reads: 5435435
total bases: 782702640
Q20 bases: 760416905(97.1527%)
Q30 bases: 729880479(93.2513%)

Read1 after filtering:
total reads: 5407042
total bases: 778522031
Q20 bases: 757048248(97.2417%)
Q30 bases: 726878853(93.3665%)

Filtering result:
reads passed filter: 5407042
reads failed due to low quality: 8721
reads failed due to too many N: 3233
reads failed due to too short: 16439
reads with adapter trimmed: 31512
bases trimmed due to adapters: 2456432

Duplication rate (may be overestimated since this is SE data): 66.0801%

JSON report: CypressIslandWA_FG126.json
HTML report: CypressIslandWA_FG126.html

fastp -i CypressIslandWA_FG126.F.fq.gz -o CypressIslandWA_FG126.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG126.json -h CypressIslandWA_FG126.html 
fastp v0.20.0, time used: 54 seconds