WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3203418
total bases: 461292192
Q20 bases: 447681672(97.0495%)
Q30 bases: 429408459(93.0882%)

Read1 after filtering:
total reads: 3195931
total bases: 460212330
Q20 bases: 447087755(97.1481%)
Q30 bases: 428995448(93.2169%)

Filtering result:
reads passed filter: 3195931
reads failed due to low quality: 5538
reads failed due to too many N: 1949
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 67.9684%

JSON report: CypressIslandWA_FG124.json
HTML report: CypressIslandWA_FG124.html

fastp -i CypressIslandWA_FG124.F.fq.gz -o CypressIslandWA_FG124.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG124.json -h CypressIslandWA_FG124.html 
fastp v0.20.0, time used: 40 seconds