WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
TGTTCCTAACCCTGATGAGGCCGTCCCTAGTTTTGTTTCTGGTGCTATGGCTAAAGCTGG

Read1 before filtering:
total reads: 2131670
total bases: 306960480
Q20 bases: 298353465(97.1961%)
Q30 bases: 286238964(93.2495%)

Read1 after filtering:
total reads: 2113067
total bases: 304265266
Q20 bases: 295998733(97.2831%)
Q30 bases: 284083593(93.3671%)

Filtering result:
reads passed filter: 2113067
reads failed due to low quality: 3243
reads failed due to too many N: 1199
reads failed due to too short: 14161
reads with adapter trimmed: 16422
bases trimmed due to adapters: 2054070

Duplication rate (may be overestimated since this is SE data): 64.2136%

JSON report: CypressIslandWA_FG120.json
HTML report: CypressIslandWA_FG120.html

fastp -i CypressIslandWA_FG120.F.fq.gz -o CypressIslandWA_FG120.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG120.json -h CypressIslandWA_FG120.html 
fastp v0.20.0, time used: 24 seconds