WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
GCTACCGATAACAATACTGTAGGCATGGGTGATGCTGGTATTAAATCTGCCATTCAAGGC

Read1 before filtering:
total reads: 3104203
total bases: 447005232
Q20 bases: 433512593(96.9815%)
Q30 bases: 415316524(92.9109%)

Read1 after filtering:
total reads: 3085617
total bases: 444295977
Q20 bases: 431291492(97.073%)
Q30 bases: 413335643(93.0316%)

Filtering result:
reads passed filter: 3085617
reads failed due to low quality: 5027
reads failed due to too many N: 1835
reads failed due to too short: 11724
reads with adapter trimmed: 15773
bases trimmed due to adapters: 1718511

Duplication rate (may be overestimated since this is SE data): 57.0439%

JSON report: CypressIslandWA_FG116.json
HTML report: CypressIslandWA_FG116.html

fastp -i CypressIslandWA_FG116.F.fq.gz -o CypressIslandWA_FG116.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG116.json -h CypressIslandWA_FG116.html 
fastp v0.20.0, time used: 35 seconds