WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 5216386
total bases: 751159584
Q20 bases: 728890365(97.0354%)
Q30 bases: 699103508(93.0699%)

Read1 after filtering:
total reads: 5204971
total bases: 749511570
Q20 bases: 727982336(97.1276%)
Q30 bases: 698472476(93.1904%)

Filtering result:
reads passed filter: 5204971
reads failed due to low quality: 8385
reads failed due to too many N: 3030
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 56.0336%

JSON report: CypressIslandWA_FG114.json
HTML report: CypressIslandWA_FG114.html

fastp -i CypressIslandWA_FG114.F.fq.gz -o CypressIslandWA_FG114.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG114.json -h CypressIslandWA_FG114.html 
fastp v0.20.0, time used: 55 seconds