WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
AAACTCATCACGAACGTCAGAAGCAGCCTTATGGCCGTCAACATACATATCACCATTATC

Read1 before filtering:
total reads: 2401452
total bases: 345809088
Q20 bases: 334894343(96.8437%)
Q30 bases: 320613549(92.714%)

Read1 after filtering:
total reads: 2359547
total bases: 339728685
Q20 bases: 329655477(97.0349%)
Q30 bases: 315768572(92.9473%)

Filtering result:
reads passed filter: 2359547
reads failed due to low quality: 6631
reads failed due to too many N: 3257
reads failed due to too short: 32017
reads with adapter trimmed: 38494
bases trimmed due to adapters: 3591572

Duplication rate (may be overestimated since this is SE data): 68.393%

JSON report: CypressIslandWA_FG111.json
HTML report: CypressIslandWA_FG111.html

fastp -i CypressIslandWA_FG111.F.fq.gz -o CypressIslandWA_FG111.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG111.json -h CypressIslandWA_FG111.html 
fastp v0.20.0, time used: 23 seconds