WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
GTGAGCCGCTTAAAGCTACCAGTTATATGGCTGTTGGTTTCTATGTGGCTAAATACGTTA

Read1 before filtering:
total reads: 3038621
total bases: 437561424
Q20 bases: 424547392(97.0258%)
Q30 bases: 407145259(93.0487%)

Read1 after filtering:
total reads: 3012485
total bases: 431413881
Q20 bases: 419390466(97.213%)
Q30 bases: 402380057(93.2701%)

Filtering result:
reads passed filter: 3012485
reads failed due to low quality: 8201
reads failed due to too many N: 4071
reads failed due to too short: 13864
reads with adapter trimmed: 44300
bases trimmed due to adapters: 4377289

Duplication rate (may be overestimated since this is SE data): 70.0607%

JSON report: CypressIslandWA_FG108.json
HTML report: CypressIslandWA_FG108.html

fastp -i CypressIslandWA_FG108.F.fq.gz -o CypressIslandWA_FG108.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG108.json -h CypressIslandWA_FG108.html 
fastp v0.20.0, time used: 28 seconds