WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 5176272
total bases: 745383168
Q20 bases: 722747743(96.9632%)
Q30 bases: 692724884(92.9354%)

Read1 after filtering:
total reads: 5153502
total bases: 742101240
Q20 bases: 721103917(97.1706%)
Q30 bases: 691475820(93.1781%)

Filtering result:
reads passed filter: 5153502
reads failed due to low quality: 15545
reads failed due to too many N: 7225
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 71.6101%

JSON report: CypressIslandWA_FG107.json
HTML report: CypressIslandWA_FG107.html

fastp -i CypressIslandWA_FG107.F.fq.gz -o CypressIslandWA_FG107.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG107.json -h CypressIslandWA_FG107.html 
fastp v0.20.0, time used: 55 seconds