WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
GTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTCCTGTTGAGTTTATTGCTGCCG

Read1 before filtering:
total reads: 3067760
total bases: 441757440
Q20 bases: 427065540(96.6742%)
Q30 bases: 409312710(92.6555%)

Read1 after filtering:
total reads: 3034741
total bases: 436692406
Q20 bases: 424144817(97.1267%)
Q30 bases: 406980436(93.1961%)

Filtering result:
reads passed filter: 3034741
reads failed due to low quality: 21216
reads failed due to too many N: 3949
reads failed due to too short: 7854
reads with adapter trimmed: 17343
bases trimmed due to adapters: 1438153

Duplication rate (may be overestimated since this is SE data): 67.74%

JSON report: CypressIslandWA_FG104-B.json
HTML report: CypressIslandWA_FG104-B.html

fastp -i CypressIslandWA_FG104-B.F.fq.gz -o CypressIslandWA_FG104-B.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG104-B.json -h CypressIslandWA_FG104-B.html 
fastp v0.20.0, time used: 32 seconds