WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
TCTTGCTGCTGCATTTCCTGAGCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCTC

Read1 before filtering:
total reads: 4746026
total bases: 683427744
Q20 bases: 663222690(97.0436%)
Q30 bases: 636166515(93.0847%)

Read1 after filtering:
total reads: 4710272
total bases: 677363639
Q20 bases: 658767816(97.2547%)
Q30 bases: 632214798(93.3346%)

Filtering result:
reads passed filter: 4710272
reads failed due to low quality: 14985
reads failed due to too many N: 6289
reads failed due to too short: 14480
reads with adapter trimmed: 35051
bases trimmed due to adapters: 2997061

Duplication rate (may be overestimated since this is SE data): 70.5236%

JSON report: CypressIslandWA_FG101-B.json
HTML report: CypressIslandWA_FG101-B.html

fastp -i CypressIslandWA_FG101-B.F.fq.gz -o CypressIslandWA_FG101-B.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG101-B.json -h CypressIslandWA_FG101-B.html 
fastp v0.20.0, time used: 44 seconds