WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
GCTACCGATAACAATACTGTAGGCATGGGTGATGCTGGTATTAAATCTGCCATTCAAGGC

Read1 before filtering:
total reads: 3246018
total bases: 467426592
Q20 bases: 454029269(97.1338%)
Q30 bases: 435624481(93.1963%)

Read1 after filtering:
total reads: 3222865
total bases: 464070086
Q20 bases: 451601782(97.3133%)
Q30 bases: 433469533(93.406%)

Filtering result:
reads passed filter: 3222865
reads failed due to low quality: 8141
reads failed due to too many N: 4442
reads failed due to too short: 10570
reads with adapter trimmed: 13598
bases trimmed due to adapters: 1542013

Duplication rate (may be overestimated since this is SE data): 66.864%

JSON report: CatalinaCA_Q351.json
HTML report: CatalinaCA_Q351.html

fastp -i CatalinaCA_Q351.F.fq.gz -o CatalinaCA_Q351.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CatalinaCA_Q351.json -h CatalinaCA_Q351.html 
fastp v0.20.0, time used: 31 seconds