WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
GCCAGCAATAGCACCAAACATAAATCACCTCACTTAAGTGGCTGGAGACAAATAATCTCT

Read1 before filtering:
total reads: 4018777
total bases: 578703888
Q20 bases: 561747637(97.07%)
Q30 bases: 538374805(93.0311%)

Read1 after filtering:
total reads: 3978905
total bases: 572625310
Q20 bases: 556868649(97.2483%)
Q30 bases: 533913311(93.2396%)

Filtering result:
reads passed filter: 3978905
reads failed due to low quality: 10228
reads failed due to too many N: 5258
reads failed due to too short: 24386
reads with adapter trimmed: 52845
bases trimmed due to adapters: 3844311

Duplication rate (may be overestimated since this is SE data): 66.4771%

JSON report: CatalinaCA_Q332.json
HTML report: CatalinaCA_Q332.html

fastp -i CatalinaCA_Q332.F.fq.gz -o CatalinaCA_Q332.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CatalinaCA_Q332.json -h CatalinaCA_Q332.html 
fastp v0.20.0, time used: 36 seconds