WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... No adapter detected for read1 Read1 before filtering: total reads: 3300384 total bases: 475255296 Q20 bases: 460533413(96.9023%) Q30 bases: 432946109(91.0976%) Read1 after filtering: total reads: 3298255 total bases: 474579803 Q20 bases: 460267858(96.9843%) Q30 bases: 432799717(91.1964%) Filtering result: reads passed filter: 3298255 reads failed due to low quality: 1158 reads failed due to too many N: 971 reads failed due to too short: 0 reads with adapter trimmed: 0 bases trimmed due to adapters: 0 Duplication rate (may be overestimated since this is SE data): 45.9578% JSON report: CatalinaCA_393.json HTML report: CatalinaCA_393.html fastp -i CatalinaCA_393.F.fq.gz -o CatalinaCA_393.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CatalinaCA_393.json -h CatalinaCA_393.html fastp v0.20.0, time used: 41 seconds