Bismark report for: /gscratch/srlab/sr320/data/oakl/zr2096_5_s1_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/oakl/zr2096_5_s1_R2_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-1.2 -p 28 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 31503281 Number of paired-end alignments with a unique best hit: 16374541 Mapping efficiency: 52.0% Sequence pairs with no alignments under any condition: 8772637 Sequence pairs did not map uniquely: 6356103 Sequence pairs which were discarded because genomic sequence could not be extracted: 5 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 4233063 ((converted) top strand) GA/CT/CT: 3961266 (complementary to (converted) top strand) GA/CT/GA: 3951322 (complementary to (converted) bottom strand) CT/GA/GA: 4228885 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 426940151 Total methylated C's in CpG context: 36700313 Total methylated C's in CHG context: 2371638 Total methylated C's in CHH context: 12917409 Total methylated C's in Unknown context: 1275922 Total unmethylated C's in CpG context: 17093166 Total unmethylated C's in CHG context: 88529768 Total unmethylated C's in CHH context: 269327857 Total unmethylated C's in Unknown context: 5433734 C methylated in CpG context: 68.2% C methylated in CHG context: 2.6% C methylated in CHH context: 4.6% C methylated in unknown context (CN or CHN): 19.0% Bismark completed in 0d 12h 29m 23s