Bismark report for: /gscratch/srlab/sr320/data/oakl/zr2096_4_s1_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/oakl/zr2096_4_s1_R2_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-1.2 -p 28 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 23970516 Number of paired-end alignments with a unique best hit: 12996066 Mapping efficiency: 54.2% Sequence pairs with no alignments under any condition: 5579671 Sequence pairs did not map uniquely: 5394779 Sequence pairs which were discarded because genomic sequence could not be extracted: 2 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3325270 ((converted) top strand) GA/CT/CT: 3162836 (complementary to (converted) top strand) GA/CT/GA: 3171400 (complementary to (converted) bottom strand) CT/GA/GA: 3336558 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 327152295 Total methylated C's in CpG context: 28027568 Total methylated C's in CHG context: 1663488 Total methylated C's in CHH context: 9884914 Total methylated C's in Unknown context: 887743 Total unmethylated C's in CpG context: 13931018 Total unmethylated C's in CHG context: 69726939 Total unmethylated C's in CHH context: 203918368 Total unmethylated C's in Unknown context: 4114265 C methylated in CpG context: 66.8% C methylated in CHG context: 2.3% C methylated in CHH context: 4.6% C methylated in unknown context (CN or CHN): 17.7% Bismark completed in 0d 9h 41m 25s