Bismark report for: /gscratch/srlab/sr320/data/oakl/zr2096_3_s1_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/oakl/zr2096_3_s1_R2_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-1.2 -p 28 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 29548753 Number of paired-end alignments with a unique best hit: 15538532 Mapping efficiency: 52.6% Sequence pairs with no alignments under any condition: 7362629 Sequence pairs did not map uniquely: 6647592 Sequence pairs which were discarded because genomic sequence could not be extracted: 2 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3905783 ((converted) top strand) GA/CT/CT: 3850463 (complementary to (converted) top strand) GA/CT/GA: 3864345 (complementary to (converted) bottom strand) CT/GA/GA: 3917939 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 424766405 Total methylated C's in CpG context: 47521990 Total methylated C's in CHG context: 2299427 Total methylated C's in CHH context: 10754385 Total methylated C's in Unknown context: 1088578 Total unmethylated C's in CpG context: 13905252 Total unmethylated C's in CHG context: 92474489 Total unmethylated C's in CHH context: 257810862 Total unmethylated C's in Unknown context: 5012717 C methylated in CpG context: 77.4% C methylated in CHG context: 2.4% C methylated in CHH context: 4.0% C methylated in unknown context (CN or CHN): 17.8% Bismark completed in 0d 11h 51m 57s