Bismark report for: /gscratch/srlab/sr320/data/oakl/zr2096_2_s1_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/oakl/zr2096_2_s1_R2_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-1.2 -p 28 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 30325606 Number of paired-end alignments with a unique best hit: 15252038 Mapping efficiency: 50.3% Sequence pairs with no alignments under any condition: 8686749 Sequence pairs did not map uniquely: 6386819 Sequence pairs which were discarded because genomic sequence could not be extracted: 3 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3851551 ((converted) top strand) GA/CT/CT: 3775231 (complementary to (converted) top strand) GA/CT/GA: 3775326 (complementary to (converted) bottom strand) CT/GA/GA: 3849927 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 375893936 Total methylated C's in CpG context: 35557561 Total methylated C's in CHG context: 2285641 Total methylated C's in CHH context: 17025048 Total methylated C's in Unknown context: 1564052 Total unmethylated C's in CpG context: 13982978 Total unmethylated C's in CHG context: 79870113 Total unmethylated C's in CHH context: 227172595 Total unmethylated C's in Unknown context: 5094985 C methylated in CpG context: 71.8% C methylated in CHG context: 2.8% C methylated in CHH context: 7.0% C methylated in unknown context (CN or CHN): 23.5% Bismark completed in 0d 11h 29m 21s