Bismark report for: /gscratch/srlab/sr320/data/oakl/zr2096_1_s1_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/oakl/zr2096_1_s1_R2_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-1.2 -p 28 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 28603346 Number of paired-end alignments with a unique best hit: 8278708 Mapping efficiency: 28.9% Sequence pairs with no alignments under any condition: 17321475 Sequence pairs did not map uniquely: 3003163 Sequence pairs which were discarded because genomic sequence could not be extracted: 1 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2087627 ((converted) top strand) GA/CT/CT: 2089422 (complementary to (converted) top strand) GA/CT/GA: 2053204 (complementary to (converted) bottom strand) CT/GA/GA: 2048454 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 200749482 Total methylated C's in CpG context: 16776198 Total methylated C's in CHG context: 1548299 Total methylated C's in CHH context: 15644588 Total methylated C's in Unknown context: 1577296 Total unmethylated C's in CpG context: 7085155 Total unmethylated C's in CHG context: 37129478 Total unmethylated C's in CHH context: 122565764 Total unmethylated C's in Unknown context: 3382097 C methylated in CpG context: 70.3% C methylated in CHG context: 4.0% C methylated in CHH context: 11.3% C methylated in unknown context (CN or CHN): 31.8% Bismark completed in 0d 7h 22m 23s