Bismark report for: /gscratch/srlab/sr320/data/oakl/zr2096_10_s1_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/oakl/zr2096_10_s1_R2_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-1.2 -p 28 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 17448883 Number of paired-end alignments with a unique best hit: 9281826 Mapping efficiency: 53.2% Sequence pairs with no alignments under any condition: 4524878 Sequence pairs did not map uniquely: 3642179 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2365021 ((converted) top strand) GA/CT/CT: 2269427 (complementary to (converted) top strand) GA/CT/GA: 2275081 (complementary to (converted) bottom strand) CT/GA/GA: 2372297 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 252267854 Total methylated C's in CpG context: 24800640 Total methylated C's in CHG context: 1459772 Total methylated C's in CHH context: 6704299 Total methylated C's in Unknown context: 649166 Total unmethylated C's in CpG context: 10519584 Total unmethylated C's in CHG context: 54484161 Total unmethylated C's in CHH context: 154299398 Total unmethylated C's in Unknown context: 2835983 C methylated in CpG context: 70.2% C methylated in CHG context: 2.6% C methylated in CHH context: 4.2% C methylated in unknown context (CN or CHN): 18.6% Bismark completed in 0d 7h 3m 30s