Bismark report for: /gscratch/srlab/sr320/data/oakl/zr2096_3_s1_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/oakl/zr2096_3_s1_R2_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-1.2 -p 28 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 29548753 Number of paired-end alignments with a unique best hit: 10380653 Mapping efficiency: 35.1% Sequence pairs with no alignments under any condition: 15079334 Sequence pairs did not map uniquely: 4088766 Sequence pairs which were discarded because genomic sequence could not be extracted: 1 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 5195217 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 5185435 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 262116982 Total methylated C's in CpG context: 27411098 Total methylated C's in CHG context: 4352030 Total methylated C's in CHH context: 31743267 Total methylated C's in Unknown context: 1620528 Total unmethylated C's in CpG context: 7451747 Total unmethylated C's in CHG context: 47342719 Total unmethylated C's in CHH context: 143816121 Total unmethylated C's in Unknown context: 3735520 C methylated in CpG context: 78.6% C methylated in CHG context: 8.4% C methylated in CHH context: 18.1% C methylated in unknown context (CN or CHN): 30.3% Bismark completed in 0d 4h 8m 6s