Bismark report for: /gscratch/srlab/sr320/data/oakl/zr2096_10_s1_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/oakl/zr2096_10_s1_R2_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-1.2 -p 28 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 17448883 Number of paired-end alignments with a unique best hit: 6101640 Mapping efficiency: 35.0% Sequence pairs with no alignments under any condition: 9119335 Sequence pairs did not map uniquely: 2227908 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3051408 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 3050232 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 155361061 Total methylated C's in CpG context: 14429916 Total methylated C's in CHG context: 2478416 Total methylated C's in CHH context: 18074812 Total methylated C's in Unknown context: 901290 Total unmethylated C's in CpG context: 5602290 Total unmethylated C's in CHG context: 28295170 Total unmethylated C's in CHH context: 86480457 Total unmethylated C's in Unknown context: 2099324 C methylated in CpG context: 72.0% C methylated in CHG context: 8.1% C methylated in CHH context: 17.3% C methylated in unknown context (CN or CHN): 30.0% Bismark completed in 0d 2h 27m 59s