Bismark report for: /gscratch/srlab/sr320/data/oil/20150414_trimmed_2112_lane1_NB6_NoOil_GCCAAT.fastq.gz (version: v0.19.0) Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 20811265 Number of alignments with a unique best hit from the different alignments: 1614710 Mapping efficiency: 7.8% Sequences with no alignments under any condition: 18190123 Sequences did not map uniquely: 1006432 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 807395 ((converted) top strand) CT/GA: 807315 ((converted) bottom strand) GA/CT: 0 (complementary to (converted) top strand) GA/GA: 0 (complementary to (converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 31055287 Total methylated C's in CpG context: 3687058 Total methylated C's in CHG context: 4528705 Total methylated C's in CHH context: 18535716 Total methylated C's in Unknown context: 89242 Total unmethylated C's in CpG context: 629367 Total unmethylated C's in CHG context: 1030792 Total unmethylated C's in CHH context: 2643649 Total unmethylated C's in Unknown context: 50723 C methylated in CpG context: 85.4% C methylated in CHG context: 81.5% C methylated in CHH context: 87.5% C methylated in Unknown context (CN or CHN): 63.8% Bismark completed in 0d 0h 37m 38s