Bismark report for: /gscratch/srlab/sr320/data/oil/20150414_trimmed_2112_lane1_NB11_NoOil_CAGATC.fastq.gz (version: v0.19.0) Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 31266957 Number of alignments with a unique best hit from the different alignments: 4744536 Mapping efficiency: 15.2% Sequences with no alignments under any condition: 23775958 Sequences did not map uniquely: 2746463 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 2383630 ((converted) top strand) CT/GA: 2360906 ((converted) bottom strand) GA/CT: 0 (complementary to (converted) top strand) GA/GA: 0 (complementary to (converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 98368212 Total methylated C's in CpG context: 13851042 Total methylated C's in CHG context: 15940213 Total methylated C's in CHH context: 56779026 Total methylated C's in Unknown context: 264963 Total unmethylated C's in CpG context: 1480605 Total unmethylated C's in CHG context: 3375451 Total unmethylated C's in CHH context: 6941875 Total unmethylated C's in Unknown context: 127002 C methylated in CpG context: 90.3% C methylated in CHG context: 82.5% C methylated in CHH context: 89.1% C methylated in Unknown context (CN or CHN): 67.6% Bismark completed in 0d 1h 24m 3s