Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-227_S35_L004_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-227_S35_L004_R2_001_val_2.fq.gz

Data processed at 02:53 on 2020-01-03


Alignment Stats

Sequence pairs analysed in total 13531234
Paired-end alignments with a unique best hit 5777321
Pairs without alignments under any condition 6365101
Pairs that did not map uniquely 1388812
Genomic sequence context not extractable (edges of chromosomes) 3

Cytosine Methylation

Total C's analysed 170387967
Methylated C's in CpG context 7151061
Methylated C's in CHG context 914187
Methylated C's in CHH context 3215757
Methylated C's in Unknown context 47223
Unmethylated C's in CpG context 16695256
Unmethylated C's in CHG context 35577795
Unmethylated C's in CHH context 106833911
Unmethylated C's in Unknown context 363445
Percentage methylation (CpG context) 30.0%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 2.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2853863 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 2923455 original bottom strand

Deduplication

Alignments analysed 5777318
Unique alignments 4209352
Duplicates removed 1567966
Duplicated alignments were found at 1031120 different positions

Cytosine Methylation after Extraction

Total C's analysed 86316145
Methylated C's in CpG context 3751343
Methylated C's in CHG context 464615
Methylated C's in CHH context 1765502
Unmethylated C's in CpG context 8102850
Unmethylated C's in CHG context 17545411
Unmethylated C's in CHH context 54686424
Percentage methylation (CpG context) 31.6%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 3.1%

M-Bias Plot