Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-205_S26_L004_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-205_S26_L004_R2_001_val_2.fq.gz

Data processed at 02:53 on 2020-01-03


Alignment Stats

Sequence pairs analysed in total 16541499
Paired-end alignments with a unique best hit 6323593
Pairs without alignments under any condition 8364655
Pairs that did not map uniquely 1853251
Genomic sequence context not extractable (edges of chromosomes) 3

Cytosine Methylation

Total C's analysed 172098082
Methylated C's in CpG context 7352466
Methylated C's in CHG context 973752
Methylated C's in CHH context 2227658
Methylated C's in Unknown context 41273
Unmethylated C's in CpG context 18563469
Unmethylated C's in CHG context 36797171
Unmethylated C's in CHH context 106183566
Unmethylated C's in Unknown context 362805
Percentage methylation (CpG context) 28.4%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 2.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3111821 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 3211769 original bottom strand

Deduplication

Alignments analysed 6323590
Unique alignments 4719915
Duplicates removed 1603675
Duplicated alignments were found at 963679 different positions

Cytosine Methylation after Extraction

Total C's analysed 85575964
Methylated C's in CpG context 3859626
Methylated C's in CHG context 469729
Methylated C's in CHH context 1198594
Unmethylated C's in CpG context 8425101
Unmethylated C's in CHG context 17659824
Unmethylated C's in CHH context 53963090
Percentage methylation (CpG context) 31.4%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 2.2%

M-Bias Plot