Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-194_S23_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-194_S23_L003_R2_001_val_2.fq.gz

Data processed at 02:53 on 2020-01-03


Alignment Stats

Sequence pairs analysed in total 27459153
Paired-end alignments with a unique best hit 11624042
Pairs without alignments under any condition 13557404
Pairs that did not map uniquely 2277707
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 360459395
Methylated C's in CpG context 15208121
Methylated C's in CHG context 1730704
Methylated C's in CHH context 4696700
Methylated C's in Unknown context 66894
Unmethylated C's in CpG context 35095565
Unmethylated C's in CHG context 75921624
Unmethylated C's in CHH context 227806681
Unmethylated C's in Unknown context 771714
Percentage methylation (CpG context) 30.2%
Percentage methylation (CHG context) 2.2%
Percentage methylation (CHH context) 2.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5726606 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5897436 original bottom strand

Deduplication

Alignments analysed 11624042
Unique alignments 9000946
Duplicates removed 2623096
Duplicated alignments were found at 1648237 different positions

Cytosine Methylation after Extraction

Total C's analysed 203726589
Methylated C's in CpG context 8959141
Methylated C's in CHG context 996952
Methylated C's in CHH context 2787951
Unmethylated C's in CpG context 18995825
Unmethylated C's in CHG context 41660215
Unmethylated C's in CHH context 130326505
Percentage methylation (CpG context) 32.0%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 2.1%

M-Bias Plot