Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-182_S17_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-182_S17_L003_R2_001_val_2.fq.gz

Data processed at 02:53 on 2020-01-03


Alignment Stats

Sequence pairs analysed in total 31499315
Paired-end alignments with a unique best hit 13143700
Pairs without alignments under any condition 15672601
Pairs that did not map uniquely 2683014
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 407184402
Methylated C's in CpG context 14617019
Methylated C's in CHG context 1959865
Methylated C's in CHH context 4544903
Methylated C's in Unknown context 72663
Unmethylated C's in CpG context 40997905
Unmethylated C's in CHG context 84917152
Unmethylated C's in CHH context 260147558
Unmethylated C's in Unknown context 900486
Percentage methylation (CpG context) 26.3%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 1.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 6520043 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 6623657 original bottom strand

Deduplication

Alignments analysed 13143700
Unique alignments 10266522
Duplicates removed 2877178
Duplicated alignments were found at 1635843 different positions

Cytosine Methylation after Extraction

Total C's analysed 222524486
Methylated C's in CpG context 8754862
Methylated C's in CHG context 1102130
Methylated C's in CHH context 2694418
Unmethylated C's in CpG context 21458168
Unmethylated C's in CHG context 44943458
Unmethylated C's in CHH context 143571450
Percentage methylation (CpG context) 29.0%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 1.8%

M-Bias Plot