Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-120_S32_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-120_S32_L005_R2_001_val_2.fq.gz

Data processed at 02:53 on 2020-01-03


Alignment Stats

Sequence pairs analysed in total 24140505
Paired-end alignments with a unique best hit 11050083
Pairs without alignments under any condition 10973461
Pairs that did not map uniquely 2116961
Genomic sequence context not extractable (edges of chromosomes) 3

Cytosine Methylation

Total C's analysed 343610405
Methylated C's in CpG context 11964980
Methylated C's in CHG context 1672574
Methylated C's in CHH context 2746920
Methylated C's in Unknown context 46814
Unmethylated C's in CpG context 35958013
Unmethylated C's in CHG context 69824544
Unmethylated C's in CHH context 221443374
Unmethylated C's in Unknown context 719473
Percentage methylation (CpG context) 25.0%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 1.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5486119 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5563961 original bottom strand

Deduplication

Alignments analysed 11050080
Unique alignments 9470106
Duplicates removed 1579974
Duplicated alignments were found at 825606 different positions

Cytosine Methylation after Extraction

Total C's analysed 217946429
Methylated C's in CpG context 8187042
Methylated C's in CHG context 1062536
Methylated C's in CHH context 1832611
Unmethylated C's in CpG context 21761145
Unmethylated C's in CHG context 43433409
Unmethylated C's in CHH context 141669686
Percentage methylation (CpG context) 27.3%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 1.3%

M-Bias Plot