Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-111_S29_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-111_S29_L005_R2_001_val_2.fq.gz

Data processed at 02:53 on 2020-01-03


Alignment Stats

Sequence pairs analysed in total 27014996
Paired-end alignments with a unique best hit 12312883
Pairs without alignments under any condition 12235438
Pairs that did not map uniquely 2466675
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 377453061
Methylated C's in CpG context 13097257
Methylated C's in CHG context 1893893
Methylated C's in CHH context 2999476
Methylated C's in Unknown context 59372
Unmethylated C's in CpG context 39327878
Unmethylated C's in CHG context 76742866
Unmethylated C's in CHH context 243391691
Unmethylated C's in Unknown context 784343
Percentage methylation (CpG context) 25.0%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 1.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 6118719 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 6194163 original bottom strand

Deduplication

Alignments analysed 12312882
Unique alignments 10466822
Duplicates removed 1846060
Duplicated alignments were found at 941145 different positions

Cytosine Methylation after Extraction

Total C's analysed 231214858
Methylated C's in CpG context 8681437
Methylated C's in CHG context 1156157
Methylated C's in CHH context 1936920
Unmethylated C's in CpG context 22978618
Unmethylated C's in CHG context 46067897
Unmethylated C's in CHH context 150393829
Percentage methylation (CpG context) 27.4%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 1.3%

M-Bias Plot