Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-104_S28_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-104_S28_L005_R2_001_val_2.fq.gz

Data processed at 02:53 on 2020-01-03


Alignment Stats

Sequence pairs analysed in total 30015261
Paired-end alignments with a unique best hit 13633936
Pairs without alignments under any condition 13665400
Pairs that did not map uniquely 2715925
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 418448650
Methylated C's in CpG context 15436769
Methylated C's in CHG context 2036052
Methylated C's in CHH context 3597610
Methylated C's in Unknown context 67853
Unmethylated C's in CpG context 41191940
Unmethylated C's in CHG context 85561381
Unmethylated C's in CHH context 270624898
Unmethylated C's in Unknown context 900361
Percentage methylation (CpG context) 27.3%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 1.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 6781130 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 6852805 original bottom strand

Deduplication

Alignments analysed 13633935
Unique alignments 11613745
Duplicates removed 2020190
Duplicated alignments were found at 1117791 different positions

Cytosine Methylation after Extraction

Total C's analysed 256681996
Methylated C's in CpG context 10137100
Methylated C's in CHG context 1260299
Methylated C's in CHH context 2323535
Unmethylated C's in CpG context 24263858
Unmethylated C's in CHG context 51430750
Unmethylated C's in CHH context 167266454
Percentage methylation (CpG context) 29.5%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 1.4%

M-Bias Plot