Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-226_S34_L004_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-226_S34_L004_R2_001_val_2.fq.gz

Data processed at 05:18 on 2019-12-21


Alignment Stats

Sequence pairs analysed in total 11133150
Paired-end alignments with a unique best hit 4644692
Pairs without alignments under any condition 5510557
Pairs that did not map uniquely 977901
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 142579752
Methylated C's in CpG context 6040169
Methylated C's in CHG context 756511
Methylated C's in CHH context 2622283
Methylated C's in Unknown context 41120
Unmethylated C's in CpG context 13986909
Unmethylated C's in CHG context 29876966
Unmethylated C's in CHH context 89296914
Unmethylated C's in Unknown context 314802
Percentage methylation (CpG context) 30.2%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 2.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2290844 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 2353848 original bottom strand

Deduplication

Alignments analysed 4644692
Unique alignments 3438115
Duplicates removed 1206577
Duplicated alignments were found at 766007 different positions

Cytosine Methylation after Extraction

Total C's analysed 74285747
Methylated C's in CpG context 3347989
Methylated C's in CHG context 403265
Methylated C's in CHH context 1509605
Unmethylated C's in CpG context 6780487
Unmethylated C's in CHG context 15120311
Unmethylated C's in CHH context 47124090
Percentage methylation (CpG context) 33.1%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 3.1%

M-Bias Plot