Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-187_S20_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-187_S20_L003_R2_001_val_2.fq.gz

Data processed at 05:18 on 2019-12-21


Alignment Stats

Sequence pairs analysed in total 22734395
Paired-end alignments with a unique best hit 9655534
Pairs without alignments under any condition 10981695
Pairs that did not map uniquely 2097166
Genomic sequence context not extractable (edges of chromosomes) 4

Cytosine Methylation

Total C's analysed 292287685
Methylated C's in CpG context 12549941
Methylated C's in CHG context 1572027
Methylated C's in CHH context 3841851
Methylated C's in Unknown context 60882
Unmethylated C's in CpG context 29075423
Unmethylated C's in CHG context 62324170
Unmethylated C's in CHH context 182924273
Unmethylated C's in Unknown context 633983
Percentage methylation (CpG context) 30.1%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 2.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4757885 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 4897645 original bottom strand

Deduplication

Alignments analysed 9655530
Unique alignments 7098905
Duplicates removed 2556625
Duplicated alignments were found at 1509525 different positions

Cytosine Methylation after Extraction

Total C's analysed 150742463
Methylated C's in CpG context 6936899
Methylated C's in CHG context 814250
Methylated C's in CHH context 2156222
Unmethylated C's in CpG context 13822954
Unmethylated C's in CHG context 31050905
Unmethylated C's in CHH context 95961233
Percentage methylation (CpG context) 33.4%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 2.2%

M-Bias Plot