Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-168_S11_L002_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-168_S11_L002_R2_001_val_2.fq.gz

Data processed at 05:18 on 2019-12-21


Alignment Stats

Sequence pairs analysed in total 22688467
Paired-end alignments with a unique best hit 8967128
Pairs without alignments under any condition 11891374
Pairs that did not map uniquely 1829965
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 278794431
Methylated C's in CpG context 10658385
Methylated C's in CHG context 1268483
Methylated C's in CHH context 3535297
Methylated C's in Unknown context 54743
Unmethylated C's in CpG context 29012483
Unmethylated C's in CHG context 59889529
Unmethylated C's in CHH context 174430254
Unmethylated C's in Unknown context 615881
Percentage methylation (CpG context) 26.9%
Percentage methylation (CHG context) 2.1%
Percentage methylation (CHH context) 2.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4393790 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 4573338 original bottom strand

Deduplication

Alignments analysed 8967128
Unique alignments 6492386
Duplicates removed 2474742
Duplicated alignments were found at 1499379 different positions

Cytosine Methylation after Extraction

Total C's analysed 139836026
Methylated C's in CpG context 5749134
Methylated C's in CHG context 652679
Methylated C's in CHH context 1958750
Unmethylated C's in CpG context 13380784
Unmethylated C's in CHG context 28960958
Unmethylated C's in CHH context 89133721
Percentage methylation (CpG context) 30.1%
Percentage methylation (CHG context) 2.2%
Percentage methylation (CHH context) 2.2%

M-Bias Plot