Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-103_S27_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-103_S27_L005_R2_001_val_2.fq.gz

Data processed at 05:17 on 2019-12-21


Alignment Stats

Sequence pairs analysed in total 20349659
Paired-end alignments with a unique best hit 9095592
Pairs without alignments under any condition 9572011
Pairs that did not map uniquely 1682056
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 289949317
Methylated C's in CpG context 9619421
Methylated C's in CHG context 1310642
Methylated C's in CHH context 2350411
Methylated C's in Unknown context 40612
Unmethylated C's in CpG context 31065297
Unmethylated C's in CHG context 59905410
Unmethylated C's in CHH context 185698136
Unmethylated C's in Unknown context 609374
Percentage methylation (CpG context) 23.6%
Percentage methylation (CHG context) 2.1%
Percentage methylation (CHH context) 1.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4509438 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 4586153 original bottom strand

Deduplication

Alignments analysed 9095591
Unique alignments 7835836
Duplicates removed 1259755
Duplicated alignments were found at 632529 different positions

Cytosine Methylation after Extraction

Total C's analysed 183501406
Methylated C's in CpG context 6658001
Methylated C's in CHG context 852229
Methylated C's in CHH context 1574350
Unmethylated C's in CpG context 18809270
Unmethylated C's in CHG context 36884209
Unmethylated C's in CHH context 118723347
Percentage methylation (CpG context) 26.1%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 1.3%

M-Bias Plot