Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-44_S41_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-44_S41_L005_R2_001_val_2.fq.gz

Data processed at 05:39 on 2019-12-21


Alignment Stats

Sequence pairs analysed in total 13762658
Paired-end alignments with a unique best hit 6084805
Pairs without alignments under any condition 6118468
Pairs that did not map uniquely 1559385
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 170813493
Methylated C's in CpG context 8105964
Methylated C's in CHG context 922535
Methylated C's in CHH context 2562650
Methylated C's in Unknown context 43087
Unmethylated C's in CpG context 16050613
Unmethylated C's in CHG context 35418280
Unmethylated C's in CHH context 107753451
Unmethylated C's in Unknown context 349691
Percentage methylation (CpG context) 33.6%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 2.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3020647 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 3064157 original bottom strand

Deduplication

Alignments analysed 6084804
Unique alignments 4031044
Duplicates removed 2053760
Duplicated alignments were found at 1305099 different positions

Cytosine Methylation after Extraction

Total C's analysed 79983322
Methylated C's in CpG context 3855731
Methylated C's in CHG context 431899
Methylated C's in CHH context 1321843
Unmethylated C's in CpG context 7250942
Unmethylated C's in CHG context 16319796
Unmethylated C's in CHH context 50803111
Percentage methylation (CpG context) 34.7%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 2.5%

M-Bias Plot