Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-209_S29_L004_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-209_S29_L004_R2_001_val_2.fq.gz

Data processed at 05:39 on 2019-12-21


Alignment Stats

Sequence pairs analysed in total 29638248
Paired-end alignments with a unique best hit 12390019
Pairs without alignments under any condition 14317340
Pairs that did not map uniquely 2930889
Genomic sequence context not extractable (edges of chromosomes) 5

Cytosine Methylation

Total C's analysed 367398872
Methylated C's in CpG context 15426958
Methylated C's in CHG context 1845531
Methylated C's in CHH context 4197293
Methylated C's in Unknown context 75507
Unmethylated C's in CpG context 38606002
Unmethylated C's in CHG context 78585196
Unmethylated C's in CHH context 228737892
Unmethylated C's in Unknown context 791989
Percentage methylation (CpG context) 28.6%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 1.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 6084990 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 6305024 original bottom strand

Deduplication

Alignments analysed 12390014
Unique alignments 8945509
Duplicates removed 3444505
Duplicated alignments were found at 2000861 different positions

Cytosine Methylation after Extraction

Total C's analysed 181303125
Methylated C's in CpG context 8133825
Methylated C's in CHG context 907176
Methylated C's in CHH context 2249034
Unmethylated C's in CpG context 17357263
Unmethylated C's in CHG context 37254559
Unmethylated C's in CHH context 115401268
Percentage methylation (CpG context) 31.9%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 1.9%

M-Bias Plot