Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-206_S27_L004_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-206_S27_L004_R2_001_val_2.fq.gz

Data processed at 05:39 on 2019-12-21


Alignment Stats

Sequence pairs analysed in total 20931018
Paired-end alignments with a unique best hit 8694089
Pairs without alignments under any condition 9860951
Pairs that did not map uniquely 2375978
Genomic sequence context not extractable (edges of chromosomes) 2

Cytosine Methylation

Total C's analysed 247081316
Methylated C's in CpG context 10213182
Methylated C's in CHG context 1350781
Methylated C's in CHH context 3265902
Methylated C's in Unknown context 56380
Unmethylated C's in CpG context 26443083
Unmethylated C's in CHG context 53493463
Unmethylated C's in CHH context 152314905
Unmethylated C's in Unknown context 526689
Percentage methylation (CpG context) 27.9%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 2.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4266342 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 4427745 original bottom strand

Deduplication

Alignments analysed 8694087
Unique alignments 6737676
Duplicates removed 1956411
Duplicated alignments were found at 1112526 different positions

Cytosine Methylation after Extraction

Total C's analysed 128475689
Methylated C's in CpG context 5690111
Methylated C's in CHG context 686060
Methylated C's in CHH context 1836981
Unmethylated C's in CpG context 12491913
Unmethylated C's in CHG context 26714902
Unmethylated C's in CHH context 81055722
Percentage methylation (CpG context) 31.3%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 2.2%

M-Bias Plot